FUTURE PROSPECTS OF ENZYME ENGINEERING AND ENZYME TECHN...
May 28, 2011 :: Posted by - maureen :: Category - Bio Technology
Future prospects of enzyme engineering Enzyme engineering is the recent technology growing rapidly due to its higher application in a lot of fields and due to having bright and clear future vision. A most exciting development over the last few years is the application of genetic engineering techniques to enzyme technology. There are a number of properties which may be improved or altered by genetic engineering including the yield and kinetics of the enzyme, the ease of downstream processing and various safety aspects. Enzymes from dangerous or unapproved microorganisms and from slow-growing or limited plant or animal tissue may be cloned into safe high-production microorganisms. The amount of enzyme produced by a microorganism may be increased by increasing the number of gene copies that code for it. For example; The engineered cells, aided by the plasmid amplification at around 50 copies per cell, produce penicillin – G – Amidase constitutively and in considerably higher quantities than does the fully induced parental strain. Such increased yields are economically relevant not just for the increased volumetric productivity but also because of reduced downstream processing costs, the resulting crude .. more» 


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